Composite

Part:BBa_K2557017:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-EF1α promoter-TP901


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1160
    Illegal BamHI site found at 499
    Illegal BamHI site found at 2104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 748
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1357


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The EF1α promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of TP901 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References